Applicability of DNA TyperTM 19 Kit to Distinguish European, African and East Asian Population
MA Quan1, JIANG Li1, ZHAO Hui1, HUANG Meisha1, 2, DENG Pan1, 3, LI Jiuling1, WANG Ling1, MA Xin1, YE Jian1, *, LI Caixia1, *
1. Institute of Forensic Science, Ministry of Public Security & Beijing Engineering Research Center of Crime Scene Evidence Examination & National Engineering Laboratory for Forensic Science, Beijing 100038, China;
2. Shanxi Medical University, Taiyuan 030001, China;
3. Kunming Medical University, Kunming 650500, China
Abstract：Objective To investigate the applicability of DNA TyperTM 19 kit to distinguish European, African and East Asian population. Methods 563 unrelated individuals’ DNA samples, among which 199 pieces are of European, 188 of African and 176 of East Asian, were co-amplified with DNA TyperTM 19 kit to have their 18 STR loci cloned. The amplified PCR products were capillary-electrophoresed through 3130XL genetic analyzer, together with their genotyping results being analyzed by Genemapper ID v3.2. Results 197 alleles were identified from the European, with their allelic frequencies ranging from 0.003-0.568; 193 alleles were found from the African, unveiling their allelic frequencies spanning 0.003-0.436; 187 alleles were discovered from the East Asian, bestriding their allelic frequencies over 0.003-0.540. The TDP was greater than 99.999 999 999 9% for all the three populations of European, African and East Asian. Conclusions The DNA TyperTM 19 kit can be used to analyze genetic polymorphism into European, African and East Asian populations.
马泉, 江丽, 赵慧, 黄美莎, 邓盼, 李玖玲, 王玲, 马新, 叶健, 李彩霞. DNA TyperTM 19试剂盒在欧洲、非洲和东亚人群中的适用性[J]. 刑事技术, 2019, 44(4): 327-331.
MA Quan, JIANG Li, ZHAO Hui, HUANG Meisha, DENG Pan, LI Jiuling, WANG Ling, MA Xin, YE Jian, LI Caixia. Applicability of DNA TyperTM 19 Kit to Distinguish European, African and East Asian Population. Forensic Science And Technology, 2019, 44(4): 327-331.
2019, Vol. 44 
