大庆地区汉族群体18个STR基因座遗传多态性

魏福军, 赵兴春, 张建, 王天华, 贾世友, 高靖宇, 王娜

刑事技术 ›› 2014, Vol. 39 ›› Issue (6) : 44-46.
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大庆地区汉族群体18个STR基因座遗传多态性

  • 魏福军1, 赵兴春2, 张建2, 王天华1, 贾世友1, 高靖宇1, 王娜1
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Polymorphism of 18 short tandem repeat loci in han population of daqing district

  • WEI Fu-jun, ZHAO Xing-chun, ZHANG Jian, WANG Tian-hua, JIA Shi-you, GAO Jing-yu, WANG Na
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摘要

目的 调查大庆地区汉族人群18个STR基因座的遗传多态性。方法 应用DNATyperTM19试剂盒检测221名大庆地区汉族无关个体18个STR基因座的等位基因频率,并应用统计软件计算群体遗传学参数。结果 18个STR 基因座的基因型分布均符合Hardy-Weinberg平衡(P > 0.05),18个基因座的杂合度(H)在0.608~0.896之间,匹配概率(Pm)在0.016~0.196之间,个体识别概率(DP)在0.804~0.984之间,多态信息含量(PIC)在0.560~0.910之间,非父排除概率(PE)值在0.301~0.788之间。结论 18个STR基因座在大庆汉族人群中有较高的多态性,所得的群体遗传学数据可为大庆地区刑事案件的法医学的个体识别、亲权鉴定和群体遗传学研究提供基础数据。

Abstract

Objective To investigate the polymorphism of 18 STR loci in Han population from Daqing district. Methods DNATyperTM19 kit was used to detect the allelic frequencies of 18 STR loci in 221 unrelated Daqing Han individuals, and then the population genetics parameters were calculate by using statistic software. Results The results demonstrated that all loci were found to be no deviation from Hardy-Weinberg equilibrium (P>0.05), Heterozygosity (H) ranged from 0.608 to 0.896, matching probability (Pm) ranged from 0.016 to 0.196, power of discrimination (DP) ranged from 0.804 to 0.984, polymorphism information content (PIC) ranged from 0.560 to 0.910, power of exclusion (PE) ranged from 0.301 to 0.788. Conclusions The 18 STR loci are relatively abundant in polymorphic information and suitable for forensic personal identification and paternity testing.

关键词

DNATyperTM19试剂盒 / STR基因座 / 遗传多态性 / 汉族

Key words

DNATyperTM19 kit / STR loci / polymorphism / Han population

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魏福军, 赵兴春, 张建, 王天华, 贾世友, 高靖宇, 王娜. 大庆地区汉族群体18个STR基因座遗传多态性. 刑事技术. 2014, 39(6): 44-46
WEI Fu-jun, ZHAO Xing-chun, ZHANG Jian, WANG Tian-hua, JIA Shi-you, GAO Jing-yu, WANG Na. Polymorphism of 18 short tandem repeat loci in han population of daqing district. Forensic Science and Technology. 2014, 39(6): 44-46

参考文献

[1] 张建,姜成涛,赵兴春,等. DNATyper TM 15试剂盒直接扩增检验纸质样本的研究[J].刑事技术,2011(4):33-35.
[2] S.T.Kalinowski,M.L.Taper,T.C.Marshall. Revising how the computer program CERVUS accommodates genotyping error increases success in paternity assignment[J]. Mol.Ecol,2007,16(5):1006-1099.
[3] Power Stats. A computer program for the analysis of population statistics,free program distributed by the authors over the internet from http://www.promega. com/geneticid tools/.1999.
[4] 郑秀芬.法医DNA 分析[M]. 北京:中国人民公安大学出版社,2002:385-387.

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