HPLC-MS/MS同时检测全血中佐匹克隆、唑吡坦和扎来普隆

吴海, 晏晓军, 高汨, 尹坚英, 徐连生

刑事技术 ›› 2013, Vol. 38 ›› Issue (2) : 22-24.
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HPLC-MS/MS同时检测全血中佐匹克隆、唑吡坦和扎来普隆

  • 吴海, 晏晓军, 高汨, 尹坚英, 徐连生
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Simultaneously determination of zopiclone, zolpidem and zaleplon in whole blood samples by HPLC-MS/MS

  • WU Hai, YAN Xiao-jun, GAO Mi, et al
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摘要

目的 建立全血中佐匹克隆、唑吡坦和扎来普隆的液相色谱-四级杆飞行时间串联质谱联用同时检测方法。方法 采用液液萃取进行提取,提取物以Zorbax Eclipse Plus C18(2.1×50mm,1.8μm)色谱柱分离,以10mmol/L甲酸铵(含0.1%甲酸)-乙腈为流动相梯度洗脱,流速为0.2mL/min,四级杆-飞行时间串联质谱检测。结果 全血中佐匹克隆和扎来普隆的线性范围为10ng/mL~500ng/mL,检出限为3ng/mL;唑吡坦的线性范围为3 ng/mL~300 ng/mL,检出限为1ng/mL。结论 本方法准确、快速、灵敏,可用于全血中佐匹克隆、唑吡坦和扎来普隆的同时定性、定量检测。

Abstract

Objective To establish a HPLC-QTOF MS/MS method for simultaneously determination of zopiclone, zolpidem and zaleplon in whole blood samples.Methods Samples were extracted by liquid-liquid extraction. The extracts were analyzed with a Zorbax Eclipse Plus C18(2.1×50mm,1.8μm)column and gradient elution by mobile phase consisted of 10mmol/L ammonium formate(contain 0.1% formic acid )-acetonitrile at 0.2mL/min, quadrupole-time of flight tandem MS detector was used.Results The calibration curves were linear over 10ng/mL~500ng/mL for zopiclone and zaleplon, 3ng/mL~300ng/mL for zolpidem. The limit of detection was 3ng/mL for zopiclone and zaleplon, 1ng/mL for zolpidem.Conclusion The method is simple, accurate, sensitive and convenient for simultaneously qualitative and quantitative determination of zopiclone, zolpidem and zaleplon in whole blood samples.

关键词

佐匹克隆 / 唑吡坦 / 扎来普隆 / 四级杆-飞行时间串联质谱

Key words

zopiclone / zolpidem / zaleplon / Q-TOF MS/MS

引用本文

导出引用
吴海, 晏晓军, 高汨, 尹坚英, 徐连生. HPLC-MS/MS同时检测全血中佐匹克隆、唑吡坦和扎来普隆. 刑事技术. 2013, 38(2): 22-24
WU Hai, YAN Xiao-jun, GAO Mi, et al. Simultaneously determination of zopiclone, zolpidem and zaleplon in whole blood samples by HPLC-MS/MS. Forensic Science and Technology. 2013, 38(2): 22-24

参考文献

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基金

公安部应用创新计划项目[No.2005YYCXHNST092]

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