目的通过对签字笔字迹成分进行检验，达到对签字笔成分进行分离并对签字笔种类进行鉴定的目的。方法使用高效液相色谱法对蓝色签字笔字迹色痕中的色料成分进行分析，并依据分析结果对墨水的种类划分，同时考察了纸张的背景影响、最小用样量以及实验结果的重现性。结果22种蓝色签字笔样品均可以实现区分。结论本研究对实际应用有较大参考价值。

This paper presented a study on the separation of dye mixture in rollerball pen inks and identification of the sorts of ink samples. The components in blue rollerball pen inks were analyzed by HPLC, samples were divided into different categories according to the separation results; 22 samples were successfully distinguished. Meanwhile, the effect of paper materials, the limit of detection and the repeatability of the results were also explored.