应用分子生物学技术鉴定体液斑迹组织来源的研究进展

doi:10.16467/j.1008-3650.2018.01.010

刑事技术 ›› 2018, Vol. 43 ›› Issue (1) : 47-52. DOI: 10.16467/j.1008-3650.2018.01.010

综述

应用分子生物学技术鉴定体液斑迹组织来源的研究进展

孙启凡¹, 李冉冉^{1, 2}, 胡胜¹, 李彩霞¹, 叶健¹, 季安全^{1, *}

作者信息 +

Progress and Prospect of Identifying Body Fluid Source by Molecular Biology Approaches

SUN Qifan¹, LI Ranran^{1, 2}, HU Sheng¹, LI Caixia¹, YE Jian¹, JI Anquan^{1, *}

Author information +

History +

摘要

对现场生物物证进行鉴定,获取最多信息是法医物证工作者的责任和义务。过去,以DNA为基础的STR个体识别以及DNA来源人特征刻画技术都已经完全或正在逐渐被法庭科学接受和认可。而对生物物证进行组织属性鉴定也正愈益显示其重要价值,因为这有助于案件现场重建和案情回溯。本文对用分子生物学手段解决组织属性鉴定的理论基础、研究进展以及未来研究的可能方向进行综述,以期为相关研究人员提供参考意见。

Abstract

DNA profiling of short tandem repeats (STR) has always played a great important role for criminal investigation into personal identification and paternity testing. However, it is also important to mine as much information as possible from biological evidence, other than to just present evidence for placing a suspect at a crime scene, e.g., reconstructing the crime scene by the identity of different body fluids (or their stains). Traditional body fluid identification makes use of biochemical and spectroscopic measurement, having various limitations on the items of time consumption, intensive labor, low sensitivity and doubtful specificity. Thanks to the development of molecular biology, new genetic biomarkers have been studied and proposed for forensic identification of body fluids into MicroRNA (miRNA), messenger RNA (mRNA), DNA methylation and microbial species. In this review, an overview was provided on the molecular markers to identify body fluid origin, together with the expatiation on the latest research progress and applications.

导出引用

孙启凡, 李冉冉, 胡胜, 李彩霞, 叶健, 季安全. 应用分子生物学技术鉴定体液斑迹组织来源的研究进展. 刑事技术. 2018, 43(1): 47-52 https://doi.org/10.16467/j.1008-3650.2018.01.010

SUN Qifan, LI Ranran, HU Sheng, LI Caixia, YE Jian, JI Anquan. Progress and Prospect of Identifying Body Fluid Source by Molecular Biology Approaches. Forensic Science and Technology. 2018, 43(1): 47-52 https://doi.org/10.16467/j.1008-3650.2018.01.010

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