CHENG Wen-bin，DONG Ying-chun，MIN Jian-xiong
Forensic Science And Technology. 2010, 0(4): 25.
Abstract (
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Objective To explore the specificity of Actin、Dm、Fn and Mb for postmortem diagnosis of human AMI in fo— rensic practice．Methods 30 autopsy hearts which had been diagnosed in forensic practice were divided into three groups：AMI， coronary artery sclerosis and normal myocardium,Tissue blocks were selected from the region of left ventricle．Five sections were cut off and stained for HE，Actin、Dm、Fn and Mb using IHC streptavidin-biotin-peroxidase technique．Image analysis technique was applied tO detect and compare intensity of IHC stain in each group．Results In AMI and coronary artery sclerosis group，the deposit of Actin、Dm andMb in myocytes was poor，but the deposit of Fn was obvious and deposit between the two groups were not significant difference．Moreover，in the normal myocardium group，the deposit of Actin、Dm and Mb in myoeytes is of no absence， but the deposit of Fn was noughL Conclusions Actin、Dm、Fn and Mb can detect the developed myocardial necrosis with IHC technique，but can’t differentiate the causes of them．The poor specificity of Actin、Dm、Fn and Mb exhibiting in ourstudy suggests that we should be all—sided considered when we apply the IHCmarker for the postmortem diagnosis of AMI in forensic practice．
