25 October 2012, Volume 37 Issue 5

    

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Orginal Article
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  Application of Laser Capture Microscope and low volume PCR in forensic DNA analysis

  ZENG Xian-hai, LI Bin

  Forensic Science And Technology. 2012, 37(5): 3-5.

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  Objective To establish a new method for reducing the bubbles in process of Low Volume PCR (LV-PCR).Methods Target cells were collected under Laser Capture Microscope and LV-PCR was performed.Results Bubbles can be reduced by skillful collection operations and LV-PCR amplification.Conclusion The LV-PCR method was better than standard PCR method for analysis of varying concentration DNA samples.
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  Development of latent blood fingerprints by water-soluble CdTe quantum dots

  CAI Kai-yang, YANG Rui-qin, WANG Yan-ji

  Forensic Science And Technology. 2012, 37(5): 6-8.

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  Objective To explore a new method for developing latent blood fingerprints with the water-soluble cadmium telluride quantum dots on various surfaces.Methods Blood fingerprints were treated with quantum dots in aqueous solution and fluorescence images were obtained under 365nm UV light.Results The develop time was less than other QDs methods reported before. Compared to TMB and acid black 10B, excellent improvement of image quality and sensitivity were also obtained.Conclusion The water-soluble cadmium telluride quantum dots method can be used to detect blood fingerprints on various surfaces.
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  Genetic analysis of the partial hydatidiform mole in a rape case

  YUAN Mei-qing, ZHOU Yi, SHI Yi, WEI Fang-nian, LI Cai-xia, LI Wan-shui

  Forensic Science And Technology. 2012, 37(5): 9-11.

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  Objective To discuss the result of DNA typing for a partial hydatidiform mole sample in a rape case.Methods An abortus tissue sample and the blood samples of the suspect and victim were analyzed by multiple STR typing with DNA TyperTM 15 kit.Results The STR genotype of this abortus tissue sample showed three peaks of which two comes from the suspect and the other comes from the victim. The result suggested that it's a partial hydatidiform mole which DNA comes from one egg and two sperms.Conclusion There are different hydatidiform moles that STR genotyping could determine the DNA resource and fertilization types. The STR genotyping results could also provide the proof of paternity testing and criminal case inquisition.
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  Underlying data recovery of DVR videos based on keyworks-searching

  YAO Bo, JIA Yong-sheng, SONG Run

  Forensic Science And Technology. 2012, 37(5): 12-14.

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  Objective To establish a method for recovering DVR video file.Methods The documents head, tail sign were determined by keyword search method first, and then the underlying data of video files was recovered.Results The underlying data of the DVR video file was recovered successfully.Conclusion The keyword search method is effective in the underlying data recovery of DVR video file.
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  Analysis on watermark characteristics of counterfeit RMB currency

  SUN Hua-qing, LIANG Lu-ning, ZHANG Ling-fei, TIAN Li-li, LIN Lei-xiang

  Forensic Science And Technology. 2012, 37(5): 15-17.

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  Objective To establish a method for identify the watermark characterisitics counterfeit RMB currency.Methods The forge watermark on counterfeit RMB were observed under UV light and transmitting light.Results There are significant different between forged watermark and real watermark, such as printing mode, printing process and morphological characteristics.Conclusion The classified characteristics of forged watermark can be used to trace clues in investigation of counterfeit currency cases.
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  Fast recognition of gasoline grades by using transportable m-fast GC E-Nose

  FU De-feng, SHEN Wei-dong, MOU Wei-min, XUAN Yu, HAN Bin-long

  Forensic Science And Technology. 2012, 37(5): 18-21.

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  The fast recognition method of gasoline grades by transportable m-fast GC E-Nose is presented. It includes pretreatment, feature extraction and classification modeling. The experimental results showed that capabilities of recognition methods using principal component analysis (PCA) are quite good and the satisfied classification performance can be obtained through selecting proper PCA factors, so this method can be used in fast recognition of gasoline grades.
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  Analysis of nimesulide and dioxopromethazine in biological sample by GC/MS

  ZHANG Zhao-hong, LIU Chun-fang, LI Guo-qing

  Forensic Science And Technology. 2012, 37(5): 22-24.

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  Objective To develop a GC/MS method for determination of nimesulide and dioxopromethazine from biological sample.Methods The nimesulide and dioxopromethazine were extracted by immersion method, qualitative analyzed by GC/MS and quantitative analyzed by GC/MS with selected ion monitoring mode (SIM).Results The lowest recovery rate was 69%, the linear correlation coefficient of nimesulide was 0.998, the linear correlation coefficient of dioxopromethazine was 0.9973.Conclusion The method is easy and reliable.
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  Development of latent fingerprint by fluorescent yellow wet-powder

  ZHANG Xiao-mei

  Forensic Science And Technology. 2012, 37(5): 25-27.

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  Fingerprints on non-porous and semi-porous surfaces were developed with fluorescent yellow wet-powder experimentally and the results showed that fingerprints on non-porous and semi-porous surfaces can be developed with good contrast and bright fluoresce. The fluorescent yellow wet-powder method is very effective for developing the latent perspiration, oil and blood fingerprints deposited on non-porous and semi-porous surfaces.
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  Automated identification of common organic toxicants by GC/MS/AMDIS

  LI Qian, HUANG Wei, LAI Wen-Bin, DAI Wei-lie, WEN Jin-feng, WANG Song-cai, ZHANG Xiao-ting

  Forensic Science And Technology. 2012, 37(5): 28-31.

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  Objective To establish an automatic GC/MS/AMDIS screening method of common organic toxicants.Methods A mass spectra library of common toxicants was built; the separate parameters and search parameters were optimized and then the toxicant standards were analyzed with AMDIS.Results an AMDIS target mass spectra library containing 2344 toxicants were built; the RT and LOD of 162 common toxicants were measured.Conclusion GC/MS/AMDIS screening method is a quick, sensitive and reliable method for familiar toxicants and can be used for rapid screening of common organic toxicants.
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  Experimental research on the sequence between laser printing and gel pen writing without intersecting strokes

  WANG Shao-fang

  Forensic Science And Technology. 2012, 37(5): 32-34.

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  Objective To explore the characteristics of different sequences of laser printing and gel pen writing without intersecting strokes.Methods Two groups of document were made by laser printing and volunteers' handwriting in different sequences, and then the documents were observed with Optem A-Zoom 2 video topography microscope.Results There were at least five classes of feature differentiae in different sequences of laser printing and gel pen writing without intersecting strokes.Conclusion These differentiae provided base for determination of sequence between laser printing and gel pen writing without intersecting strokes.
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  Preliminary study on the detection of blood fingerprint on no-porous surface by Acid Yellow 7

  SHUI Jing-jing, LUO Ya-ping, WANG Ming-chao

  Forensic Science And Technology. 2012, 37(5): 35-37.

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  Objective To introduce a new method to develop blood fingerprints on no-porous surface by a protein dye.Methods Blood fingerprints deposited on ceramic tile, black plastic bag, ring-pull can were developed with Acid Yellow 7, and observed under alterative light source.Results Viewed through the orange goggles, the blood fingerprints developed by acid yellow 7 exhibited yellow colour under the natural light and photoluminescence with excitation of 440 nm light.Conclusion This method is easy to operate and effective.
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  Examination of permanent MAC address and current MAC address of ethernet NIC

  XU Lian, ZHANG Guo-chen

  Forensic Science And Technology. 2012, 37(5): 38-40.

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  This paper reviewed examination methods of MAC Address,including the MAC permanent address and current address, and introduced feasible ways to conduct examination on various of Ethernet NIC. The author suggested that the more attention should be pay on development of online examination methods of MAC Address at crime scene.
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  A reviewing for distribution and postmortem redistribution of Ketamine

  HUANG Ming, ZHOU An-ju, SUN Lin-feng, ZHANG Sheng

  Forensic Science And Technology. 2012, 37(5): 41-43.

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  As an important dissociative anesthesia drug, ketamine was widely used in clinic field. Recently the problem of ketamine abuse as well as the drug-facilitated crimes is increasingly emerging. In order to provide the basis and research considerations, the distribution and postmortem redistribution of ketamine and the effect factors such as temperature, storage condition, multi-drug effect and interaction among adjacent tissues or organs were specifically demonstrated in this paper.