Ligase chain reaction assay for gender typing test of human Amelogenin locus

GONG Rong-bin, LIU Kang-wu, MIAO Ming, et al

Forensic Science and Technology ›› 2013, Vol. 38 ›› Issue (1) : 35-37.

Ligase chain reaction assay for gender typing test of human Amelogenin locus

  • GONG Rong-bin, LIU Kang-wu, MIAO Ming, et al
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Abstract

Objective To explore the forensic application of Ligase Chain Reaction (LCR).Methods The human Amelogenin locus was selected for LCR. According to the sequences of M55418 on the human Amelogenin chromosome loci Xp22.1-3, and M55419 on Yp11.2, the primers was designed and the LCR system was set up for genotyping male and female.Results The genotypes of male and female Amelogenin loci can be get by nested LCR correctly.Conclusion LCR can be applied to human Amelogenin locus genotyping.

Key words

ligase chain reaction(LCR) / Amelogenin lOCUS

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GONG Rong-bin, LIU Kang-wu, MIAO Ming, et al. Ligase chain reaction assay for gender typing test of human Amelogenin locus. Forensic Science and Technology. 2013, 38(1): 35-37

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