Application of real-time quantitative PCR (SYBR Green I) technique in forensic science

SHI Yue-qun,HU Lan

Forensic Science and Technology ›› 2012, Vol. 37 ›› Issue (3) : 9-12.
Orginal Article

Application of real-time quantitative PCR (SYBR Green I) technique in forensic science

  • SHI Yue-qun,HU Lan
Author information +
History +

Abstract

Objective To apply the real-time quantitative PCR assay with the SYBR Green I to DNA analysis in forensic science. Methods DNA abstracted from variety of biological samples encountered in forensic science were quantitated by using the SYBR Green I, and the subsequent procedures of STR testing were then conducted. Results The quantity of DNA of variety of biological samples was obtained. Conclusion Real-time quantitative PCR is useful technique for DNA analysis in forensic science.

Key words

forensic biological evidence / fluorescent quantitative PCR / quantification / STR

Cite this article

Download Citations
SHI Yue-qun,HU Lan. Application of real-time quantitative PCR (SYBR Green I) technique in forensic science. Forensic Science and Technology. 2012, 37(3): 9-12

References

[1] 陈茹,孙洋,刘军,等.荧光实时定量PCR方法检测猪链球菌2型[J]. 中国兽医学报,2008(1):5-7.
[2] 王梁燕,洪其华.实时定量PCR技术及其应用[J].细胞生物学杂志.2004(2):62-67.
[3] Antonio A,Pablo M,Cristina A,et a1.Real-time PCR designs tO estimate nuclear and mitochondrial DNA copy nttmher in forensic and ancient DNA studies[J].Forensic Sci-ence International,2004,139:141-149.
[4] 郝金萍,吴巧雯,严江伟,等.定量PCR技术在法医学中应用的研究. 中国法医学杂志,2005,20(2):77-79.
[5] Heid CA,Stevens J,Livak KJ,Williams PM.Real time quantitative PCR[J].Genome Res,1996,6(10):986-994.
[6] 李成涛,林源,柳燕,等.实时DNA定量技术的应用研究[J].中国司法鉴定,2007:28-30.
[7] 徐君怡,曹际娟,王长文,等.虎制品的SYBR Green I实时荧光PCR特异性鉴定[J].东北林业大学学报,2010,38(10):82-84.
[8] 刘歆,徐根明,郭江峰,等.基于SYBR Green I的双链DNA定量方法.中国生物工程杂志,2008,28(1):55-60.

Accesses

Citation

Detail

Sections
Recommended

/