The handwriting samples collected from 236 college students (122 males and 114 females) were studied and the results can be summarized as following:The typographical quality of male is lower than that of female;the writing speed of male is faster than that of female;handwriting of male is more scribbled and the rhythm of handwriting is weaker than that of female;the margin width of handwriting of male is smaller than that of female;the stroke connections of handwriting of male is more than that of female;the turning angle and the writing spacing of handwriting of male are larger than that of female.