Objective To explore the application of small particle TiO2 reagent on development of latent fingerprints. Methods (40) Fingerprints on six different kinds of substrates, which were preserved one day and seven days, and they were developed by SPR (small particle reagent).Results The fingerprints preserved for one day and seven days have not evident difference, but the developing result of sebaceous fingerprint was better than the eccrine fingerprints. Conclusion The small TiO2 particle reagent is effective reagent for latent fingerprints on the non-porous surfaces.