Novel Approach of Ultrasonic-assisted Ionic Liquid Dispersive Liquid-liquid Microextraction Coupled with LC-MS/MS to Determine Blood-harbored Carbamazepine

doi:10.16467/j.1008-3650.2021.0110

Forensic Science and Technology ›› 2021, Vol. 46 ›› Issue (5) : 478-483. DOI: 10.16467/j.1008-3650.2021.0110

LÜ Chenxi, LU Xiaojun, LIANG Jiahao, XIE Wenkai, GUAN Qinglin, WEI Zhiwen, ZHANG Chao, DU Qiuxiang, YUN Keming, CUI Haiyan^*

Author information +

History +

Abstract

Objective To establish a novel approach of determining carbamazepine in whole blood through ultrasonic-assisted ionic liquid dispersive liquid-liquid microextraction coupled with HPLC-MS/MS (high performance liquid chromatography-tandem spectrometry). Methods With the selected 1-butyl-3-methylimidazolium hexafluoro-phosphate ([Bmim][PF6]) as extract solvent and 10,11-dihydrocarbamazepine as internal standard, the blank whole blood samples were added of carbamazepine, subjected to treating with ultrasound-assisted ionic liquid dispersive liquid-liquid microextraction. The harvested extraction was consecutively separated with ZORBAX Eclipse Plus C18 column that was flowed with mobile phases [A: 0.1% formic acid aqueous solution plus 10mM ammonium acetate and B: acetonitrile/methanol (v/v, 2:3)] running at the rate of 1mL/min. The detection was chosen under the mode of ESI⁺ plus MRM (multiple reaction monitoring). Results Carbamazepine was of linearity with it ranging from 8.00ng/mL to 300.00ng/mL in whole blood, with its correlation coefficient (R²) greater than 0.995, limit of detection (LOD) being 3.00ng/mL and limit of quantification (LOQ) 8.00ng/mL. The here-established approach was of extraction recovery greater than 80%, intra- and inter-day precision (relative standard deviation, RSD) being both less than 20%. Through verification of the here-established approach, a real forensic case was identified with carbamazepine of 2.71μg/mL in the victim's whole blood. Conclusions The here-developed approach is environmentally friendly, rapid, of good enrichment effect, high sensitivity and low consumption of organic solvent, capable of being used for determination of carbamazepine in biological samples from related forensic poisoning cases.

Key words

forensic toxicology / HPLC-MS/MS / ultrasonic-assisted ionic liquid dispersion liquid-liquid microextraction / carbamazepine / whole blood

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

LÜ Chenxi, LU Xiaojun, LIANG Jiahao, XIE Wenkai, GUAN Qinglin, WEI Zhiwen, ZHANG Chao, DU Qiuxiang, YUN Keming, CUI Haiyan. Novel Approach of Ultrasonic-assisted Ionic Liquid Dispersive Liquid-liquid Microextraction Coupled with LC-MS/MS to Determine Blood-harbored Carbamazepine. Forensic Science and Technology. 2021, 46(5): 478-483 https://doi.org/10.16467/j.1008-3650.2021.0110

References

[1] 倪青,曹岸江. 常用临床药物手册[M]. 北京:中国医学科技出版社,1999: 131-132.
(NI Qing, CAO Anjiang. Manual of commonly used clinical drugs [M]. Beijing: China Medical Science Press, 1999: 131-132.)
[2] 李惟滔,朱旭,肇丽梅. 治疗药物监测在抢救卡马西平中毒患者中的应用[J]. 中国医院用药评价与分析,2020, 20(6): 727-728.
(LI Weitao, ZHU Xu, ZHAO Limei. Therapeutic drug monitoring in the rescue of patients with carbamazepine intoxication[J]. Evaluation and Analysis of Drug-use in Hospitals of China, 2020, 20(6): 727-728.)
[3] 杨盛茹,姚喜梅,邹建,等. 超高效液相色谱串联质谱检测动物组织中卡马西平及其代谢物的残留量[J]. 现代食品科技,2018, 34(4): 221-226.
(YANG Shengru, YAO Ximei, ZOU Jian, et al. Simultaneous determination of carbamazepine and its metabolites in animal matrix using ultra performance liquid chromatography tandem mass spectrometry[J]. Modern Food Science and Technology, 2018, 34(4): 221-226.)
[4] 陈晓,朱斌,杨阳,等. UPLC-MS/MS法同时检测人血浆中拉莫三嗪和卡马西平浓度[J]. 实用药物与临床,2019, 22(10): 1083-1086.
(CHEN Xiao, ZHU Bin, YANG Yang, et al. Simultaneous determination of lamotrigine and carbamazepine in human plasma by UPLC-MS/MS[J]. Practical Pharmacy and Clinical Remedies, 2019, 22(10): 1083-1086.)
[5] ACEÑA J, PÉREZ S, EICHHORN P. Metabolite profiling of carbamazepine and ibuprofen in Solea senegalensis bile using high-resolution mass spectrometry[J]. Analytical and Bioanalytical Chemistry , 2017, 409(23): 5441-5450.
[6] SUN J N, SHI Y P, CHEN J. Ultrasound-assisted ionic liquid dispersive liquid-liquid microextraction coupled with high performance liquid chromatography for sensitive determination of trace celastrol in urine[J]. Journal of Chromatography B, 2011, 879(30): 3429-3433.
[7] MENG X, XU C, HAN Y, et al. Comprehensive analysis of chloroaluminate and composite ionic liquids using direct analy-sis in real time mass spectrometry[J]. Entia Sinica Chimica, 2020, 50(6): 720-728.
[8] 向平,沈敏,卓先义. 生物样品分析方法的有效性验证[J]. 法医学杂志,2008, 24(1): 60-65.
(XIANG Ping, SHEN Min, ZHUO Xianyi. How to validate a bioanalytical method[J]. Journal of Forensic Medicine, 2008, 24(1): 60-65)
[9] LIAO Y, HOU Y, ZHONG Y. One-step ionic liquid-based ultrasound-assisted dispersive liquid-liquid microextraction coupled with high-performance liquid chromatography for the determination of pyrethroids in traditional Chinese medicine oral liquid preparations[J]. BMC chemistry, 2019, 13(1): 1-10.
[10] 吴谦,林花, 刘迎,等. 离子液体-超声辅助-固相萃取法提取富集急性子药材中的4种凤仙萜四醇皂苷[J]. 分析化学,2018, 46(6): 910-916.
(WU Qian, LIN Hua, LIU Ying, et al. Ionic liquid ultrasonic-assisted solid phase extraction of four kinds of hosenkosides from seeds of Impatiens balsamina L[J]. Chinese Journal of Analytical Chemistry, 2018, 46(6): 910-916.)
[11] WANG S, WANG S, LIU C. Ionic liquid-based dispersive li-quid-liquid microextraction following high-performance li-quid chromatography for the determination of fungicides in fruit juices[J]. Food Analytical Methods, 2013, 6(2): 481-487.
[12] LI Q, LIU W, ZHU X. Green choline amino acid ionic liquid aqueous two-phase extraction coupled with HPLC for analysis sunset yellow in beverages[J]. Food Analytical Methods, 2019, 12(11): 2527-2534.
[13] 陈晓红,魏晓琴,蔡美强,等. 离子液体[C4mim][PF6]超声辅助萃取/LC-MS/MS法测定水中痕量溴敌隆及溴鼠灵[J]. 分析测试学报,2014, 33(6): 648-652.
(CHEN Xiaohong, WEI Xiaoqin, CAI Meiqiang, et al. Simultaneous determination of bromoadiolone and brodifacoum in water by LC-MS/MS and ultrasonic assisted extraction with ionic liquid [C4mim][PF6][J]. Journal of Instrumental Analysis, 2014, 33(6): 648-652.)
[14] 张静,袁宇琳,熊雯. UPLC-MS/MS法测定人血浆中卡马西平浓度[J]. 中国药物应用与监测,2020, 17(2): 86-89.
(ZHANG Jing, YUAN Yulin, XIONG Wen. Determination of carbamazepine in human plasma by UPLC-MS/MS[J]. Chinese Journal of Drug Application and Monitoring, 2020, 17(2): 86-89.)
[15] 毕重文,杨丽,李丹青,等. UPLC-MS/MS法检测人血清中卡马西平浓度及其临床应用[J]. 中国临床药理学杂志,2020, 36(12): 1715-1718.
(BI Chongwen, YANG Li, LI Danqing, et al. Quantitative determination of carbamazepine in human serum by UPLC-MS/MS and its clinical application[J]. The Chinese Journal of Clinical Pharmacology, 2020, 36(12):1715-1718.)
[16] 张剑萍,郭澄. 生物样品分析中的方法学验证[J]. 中国药房,2008, 19(31): 2469-2471.
(ZHANG Jianping, GUO Cheng. Bioanalytical Methodology validation[J]. China Pharmacy, 2008, 19(31): 2469-2471

Accesses

Citation

Detail

Sections

Recommended

Abstract
Key words
Cite this article
References

Received	Revised	Published
2021-01-15	2021-06-11	2021-10-15
Issue Date
2021-10-15

Please choose a citation manager

Content to export

Abstract

Key words

Cite this article

{{custom_sec.title}}

{{custom_sec.title}}

References

{{custom_fnGroup.title_en}}

Footnotes