Objective To set up one LC-MS/MS method for determination of 2′-diclazepam in blood or urine. Methods The sample of blood or urine was treated through liquid-liquid extraction to isolate the 2′-diclazepam that was afterwards subjected to LC-MS/MS. Results A good linear relationship was present between the peak area and concentration in 0.1~100ng/mL of 2′-diclazepam in blood or urine, with the limit of detection (LOD) being 0.01ng/mL and recoveries among 60.67%~89.03%. Conclusions The method is reliable, facilitating and sensitivity-high, capable of being used for determination of 2′-diclazepam in blood or urine.
Key words
forensic toxicology /
2′-diclazepam /
blood /
urine /
LC-MS/MS
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