Batch-tested DNA samples enrolled into database are important for China National DNA database to maintain and develop. The relevant testing process is characteristic of large scale and standardization, usually different from the testing of case samples in one routine forensic DNA lab. Based on the experience of long-time DNA testing of such kind and borrowing the concept of assembly-production line, this paper summarizes a series of quality-control measures about the relevant manipulations so that the sample-enrolled DNA database can assure its fidelity, with some references being also provided for setting up one systematic test standard and a relevant procedure.