DNA Direct Amplification: Its Forensic Application and Research Progress

SHEN Wei, MA Jun, PAN Haojie, DENG Mingming, LI Qiao, REN Wenyan

Forensic Science and Technology ›› 2018, Vol. 43 ›› Issue (5) : 390-395. DOI: 10.16467/j.1008-3650.2018.05.010
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DNA Direct Amplification: Its Forensic Application and Research Progress

  • SHEN Wei1, MA Jun2, PAN Haojie1, DENG Mingming1, LI Qiao1, REN Wenyan3,*
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Abstract

DNA direct amplification is of many advantages such as saving time, improving sensitivity, reducing operation steps and lowering the risk of contamination, thereby capable of economizing the workload, material expense, financial resources and time so that its development and application unfold important values for the database construction, emergent and/or high-profile cases as well as the grave disasters. Currently, DNA direct amplification has been widely used in the database construction, being also deepened/expanded of its utilization into the routine casework. This paper is to provide a comprehensive view of DNA direct amplification about its forensic researches and applications through explaining the features of the technology, introducing the application of relevant commercial kits, analyzing the practice of this technology into testing the commonly-seen/contact samples, together with the influential factors of direct PCR and rapid direct amplification. In addition, the impact is also described on the types of swab and reagents in direct amplification, and accordingly the discovery and significance of free DNA being elucidated as well.

Key words

forensic genetics / direct amplification / STR genotype

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SHEN Wei, MA Jun, PAN Haojie, DENG Mingming, LI Qiao, REN Wenyan. DNA Direct Amplification: Its Forensic Application and Research Progress. Forensic Science and Technology. 2018, 43(5): 390-395 https://doi.org/10.16467/j.1008-3650.2018.05.010

References

[1] 侯一平. 法医物证学[M]. 4版. 北京:人民卫生出版社,2016.
[2] GAUSTERER C, STEIN C, STIMPFL T.Application of direct PCR in a forensic case of yew poisoning[J]. International Journal of Legal Medicine, 2012, 126(2): 315-319.
[3] VERHEIJ S, HARTEVELD J, SIJEN T.A protocol for direct and rapid multiplex PCR amplification on forensically relevant samples[J]. Forensic Science International: Genetics, 2012, 6(2): 167-175.
[4] LINACRE A, PEKAREK V, SWARAN Y C, et al.Generation of DNA profiles from fabrics without DNA extraction[J]. Forensic Science International: Genetics, 2010, 4(2): 137-141.
[5] 张艳霞,李爱强,赵丽,等. 直接扩增法用于常见现场检材DNA检验[J]. 中国法医学杂志,2012,27(1):62-63
[6] 钱水,张勇果,杨电. PowerPlex21直接扩增法DNA检验应用[J]. 中国法医学杂志,2015 (6): 629-631.
[7] TEMPLETON J, OTTENS R, PARADISO V, et al.Genetic profiling from challenging samples: direct PCR of touch DNA[J]. Forensic Science International: Genetics Supplement Series, 2013, 4(1): 224-225.
[8] WANG D Y, CHANG C W, OLDROYD N J, et al.Direct amplification of STRs from blood or buccal cell samples[J]. Forensic Science International: Genetics Supplement Series, 2009, 2(1): 113-114.
[9] 张建,姜成涛,赵兴春,等. DNATyper™ 15试剂盒直接扩增检验纸质样本的研究[J]. 刑事技术,2011 (4): 33.
[10] OTTENS R, TEMPLETON J, PARADISO V, et al.Application of direct PCR in forensic casework[J]. Forensic Science International: Genetics Supplement Series, 2013, 4(1): 47-48.
[11] BALOGH M K, BURGER J, BENDER K, et al.STR genotyping and mtDNA sequencing of latent fingerprint on paper[J]. Forensic Science International, 2003, 137(2): 188-195.
[12] 郑小婷,徐念来. 硅珠法提取生物检材DNA的影响因素[J]. 法医学杂志,2016, 32(1): 63-64.
[13] BUTLER J M.Forensic DNA typing: biology, technology, and genetics of STR markers[M]. NewYork:Academic Press, 2005.
[14] SWARAN Y C, WELCH L.A comparison between direct PCR and extraction to generate DNA profiles from samples retrieved from various substrates[J]. Forensic Science International: Genetics, 2012, 6(3): 407-412.
[15] OHHARA M, KUROSU Y, ESUMI M.Direct PCR of whole blood and hair shafts by microwave treatment[J]. BioTechniques, 1994, 17(4): 726-728.
[16] PARK S J, KIM J Y, YANG Y G, et al.Direct STR amplification from whole blood and blood- or saliva- spotted FTA® without DNA purification[J]. Journal of Forensic Sciences, 2008, 53(2): 335-341.
[17] OTTENS R, TAYLOR D, ABARNO D, et al.Successful direct amplification of nuclear markers from a single hair follicle[J]. Forensic Science, Medicine, and Pathology, 2013, 9(2): 238-243.
[18] TEMPLETON J E, LINACRE A.DNA profiles from fingermarks[J]. BioTechniques, 2014, 57(5): 259.
[19] TIE J, UCHIGASAKI S.Detection of short tandem repeat polymorphisms from human nails using direct polymerase chain reaction method[J]. Electrophoresis, 2014, 35(21-22): 3188-3192.
[20] FLORES S, SUN J, KING J, et al.Internal validation of the GlobalFiler™ Express PCR Amplification Kit for the direct amplification of reference DNA samples on a high-throughput automated workflow[J]. Forensic Science International: Genetics, 2014, 10: 33-39.
[21] WANG D Y, CHANG C W, LAGACÉ R E, et al.Development and validation of the AmpF?STR® Identifiler® direct PCR amplification kit: A multiplex assay for the direct amplification of single - Source Samples[J]. Journal of Forensic Sciences, 2011, 56(4): 835-845.
[22] 巴华杰,林子清,刘亚楠,等. 5种免提取试剂盒检验滤纸血痕结果的比较[J]. 中国法医学杂志,2013 (1): 49-51.
[23] MYERS B A, KING J L, BUDOWLE B.Evaluation and comparative analysis of direct amplification of STRs using PowerPlex® 18D and Identifiler® direct systems[J]. Forensic Science International: Genetics, 2012, 6(5): 640-645.
[24] SIM J E, PARK S J, LEE H C, et al.High-throughput STR analysis for DNA database using direct PCR[J]. Journal of Forensic Sciences, 2013, 58(4): 989-992.
[25] BARBARO A, CORMACI P, VOTANO S.Direct PCR by the AmpFlSTR NGM™ kit for database purpose[J]. Forensic Science International: Genetics Supplement Series, 2011, 3(1): e103-e104.
[26] 刘超,冯冬亮,刘长晖. DNA数据库建设中批量样本直接扩增检验法的应用[J]. 中国法医学杂志,2010, 25(2): 79-81.
[27] 刘亚举,张俊涛, 史绍杏. 改良直接扩增法应用于生物检材DNA检验[J]. 刑事技术,2014, 39(6): 55-56.
[28] BRITO P, GOUVEIA N, BOGAS V, et al.Direct amplification of casework samples in fabrics using GlobalFiler® PCR Amplification Kit[J]. Forensic Science International: Genetics Supplement Series, 2015, 5: e487-e489.
[29] OTTENS R, TAYLOR D, ABARNO D, et al.Optimising direct PCR from anagen hair samples[J]. Forensic Science International: Genetics Supplement Series, 2013, 4(1): e109-e110.
[30] 高波,唐振亚,杨电. PowerPlex21 直扩法与磁珠法用于毛发STR检验的比较[J]. 中国公共安全(学术版),2015 (2): 111-113.
[31] DARGAY A, ROY R.Direct Y-STR amplification of body fluids deposited on commonly found crime scene substrates[J]. Journal of Forensic and Legal Medicine, 2016, 39: 50-60.
[32] 李林,刘静,王敏,等. 直接扩增法在血痕, 肋软骨和唾液检材中的应用[J]. 中国法医学杂志,2013, 28(2): 148-150.
[33] 王传海,徐程,李湘秦,等. 直接扩增法用于重大灾难事故中软骨的个体识别[J]. 法医学杂志,2017, 33(3): 281-283.
[34] 杨电,陈俊璋,李越,等. 擦拭直扩法和粘取磁珠法检测衣物脱落细胞DNA的比较[J]. 法医学杂志,2017, 33(5): 538-539.
[35] 李长征,刘海东. 直扩试剂盒检验接触DNA检材初探[J]. 中国法医学杂志,2013,28(3):247.
[36] WICKENHEISER R A.Trace DNA: a review, discussion of theory, and application of the transfer of trace quantities of DNA through skin contact[J]. Journal of Forensic Science, 2002, 47(3): 442-450.
[37] THOMASMA S M, FORAN D R.The influence of swabbing solutions on DNA recovery from touch samples[J]. Journal of Forensic Sciences, 2013, 58(2): 465-469.
[38] TEMPLETON J E L, TAYLOR D, HANDT O, et al. Direct PCR improves the recovery of DNA from various substrates[J]. Journal of Forensic Sciences, 2015, 60(6): 1558-1562.
[39] BOTEZATU I, SERDYUK O, POTAPOVA G, et al.Genetic analysis of DNA excreted in urine: a new approach for detecting specific genomic DNA sequences from cells dying in an organism[J]. Clinical Chemistry, 2000, 46(8): 1078-1084.
[40] 张广峰,陈松,涂政,等. 接触DNA检验成功率的影响因素探讨[J]. 刑事技术,2013, 38(3): 9-13.
[41] KITA T, YAMAGUCHI H, YOKOYAMA M, et al.Morphological study of fragmented DNA on touched objects[J]. Forensic Science International: Genetics, 2008, 3(1): 32-36.
[42] QUINONES I, DANIEL B.Cell free DNA as a component of forensic evidence recovered from touched surfaces[J]. Forensic Science International: Genetics, 2012, 6(1): 26-30.
[43] VANDEWOESTYNE M, VAN HOOFSTAT D, FRANSSEN A, et al.Presence and potential of cell free DNA in different types of forensic samples[J]. Forensic Science International: Genetics, 2013, 7(2): 316-320.
[44] 郝晓明,孙进广,苟春宝,等. 磁珠直接吸附法对体态检材游离DNA的提取[J]. 中国法医学杂志,2017, 32(4): 379-381.
[45] 董会,魏丽,张涛,等. 微量接触类生物检材的游离DNA问题分析[J]. 分子影像学杂志,2015 (3): 177-181.
[46] VERHEIJ S, HARTEVELD J, SIJEN T.A protocol for direct and rapid multiplex PCR amplification on forensically relevant samples[J]. Forensic Science International: Genetics, 2012, 6(2): 167-175.
[47] CHEN T, CATCHESIDE D E A, STEPHENSON A, et al. A rapid wire-based sampling method for DNA profiling[J]. Journal of Forensic Sciences, 2012, 57(2): 472-477.
[48] ABOUD M, OH H H, MCCORD B.Rapid direct PCR for forensic genotyping in under 25 min[J]. Electrophoresis, 2013, 34(11): 1539-1547.
[49] 刘琳,郝晓明,董会,等. AmpFLSTR Identifiler Plus试剂盒快速直接PCR扩增初探[J]. 中国司法鉴定,2014(1): 53-55.
[50] 焦志. 直接扩增法提取脱落细胞DNA[J]. 海峡科学,2015(11): 20-21.
[51] VERHEIJ S, HARTEVELD J, SIJEN T.A protocol for direct and rapid multiplex PCR amplification on forensically relevant samples[J]. Forensic Science International: Genetics, 2012, 6(2): 167-175.

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