： Objective To explore the technique and strategy about both investigation of the crime scene and DNA test for such biologically evidential materials as exfoliated cells adhered onto the glove imprints.. Methods DNA, individually extracted from four swabs of glove imprints collected in theft cases by an improved silica-bead method, was amplified by AmpFISTR Identifiler® Plus system, followed to conduct electrophoresis by 3500XL genetic analyzer, and finally undergone the genotyping by GeneMapper® ID-X software. Results DNA genotyping profile was successfully acquired from each of the four swabs. 17 cases were solved through the combination with the evidence of the DNA genotyping profile obtained above. Conclusions Glove imprints, in combination with the efficient DNA test strategy on the basis of their careful examination and extraction at crime scenes, can significantly improve DNA directivity, thereby making such biological trace-evidence exert its function effectively.