目的采用Investigator^® DIPplex试剂盒调查广西汉族､苗族中的群体遗传学数据,并评估其法医学应用价值｡方法 对广西汉族､苗族的100名无关个体的30个InDels位点进行复合扩增,统计分析各位点的频率数据､群体遗传学参数｡结果 经Bonferroni校正,30个InDels位点的分布均符合Hardy-Weinberg平衡;各位点在广西汉族､苗族人群中的累积个体识别率分别为0.999 999 999 98､0.999 999 999 97,累积非父排除率均大于 0.96｡30个InDels在广西汉族中6个位点(D111､D118､D99､D114､D64､D39)He<0.3,苗族中8个位点(D111､D118､D99､D122､D64､D81､D39､D84)He<0.3｡通过Genepop 4.2软件计算Fst值,除D84(0.080 7)外,其余位点均小于0.06｡结论 该组30个InDels位点在广西汉族､苗族人群中具有较高的多态性,可以作为现有STR检验体系的补充｡

Objective To study 30 insertion/deletion polymorphisms (InDels) of Guangxi Han and Miao population and evaluate their forensic applicability. Methods 30 InDels and amelogenin were determined with Investigator^® DIPplex in 50 unrelated individuals in the population of Han and Miao in Guangxi. Allele frequencies were measured along with other population genetics parameters. Results No significant deviation from Hardy-Weinberg equilibrium or linkage disequilibrium after Bonferroni’s correction was observed in 30 InDels loci of Guangxi Han and Miao population. The total probability of discrimination power (TDP) was 0.99999999998 and 0.99999999997 for Guangxi Han and Miao population, respectively. The cumulative probability of exclusion (CPE) was greater than 0.96 for both populations. Out of the 30 InDels loci, six of He<0.3 for Guangxi Han population localized on D111, D118, D99, D114, D64 and D39, and for Guangxi Miao population on the eight loci of D111, D118, D99, D122, D64, D81, D39 and D84. The Fst values, calculated through Genepop 4.2 software, were less than 0.06 for all loci but D84 (0.0807). Conclusions The 30 InDels are highly polymorphic in Guangxi Han and Miao population and can be used for individual identification, both supplementary and stand-alone.