和田地区哈萨克族人群23个STR基因座遗传多态性
刘胜1, 贾菲2, 刘锋2,*
1.大连瓦房店市公安局刑侦大队,大连 116300
2.辽宁省公安厅刑事技术总队,沈阳 110032
刘 锋,男,主任法医师,本科,研究方向为法医遗传学。 E-mail: lndna0121_cn@sina.com

作者简介: 刘 胜(1975—),男,辽宁瓦房店人,主检法医师,本科,研究方向为法医学。 E-mail: 2316949642@qq.com

摘要

调查新疆和田地区哈萨克族人群23个STR基因座的遗传多态性。除D13S317基因座外,其他22个STR基因座的基因分布均符合Hardy-Weinberg平衡(P>0.05)。23个基因座的TDP达1-4.53×1029,CPE达0.999 999 999 804。23个STR基因座在新疆和田地区的哈萨克族人群中有较高的多态性,所得的群体遗传学数据可为该地区的法医学个体识别、亲权鉴定提供基础数据。

关键词: 法医物证学; STR基因座; 等位基因频率; 遗传多态性; 哈萨克族
中图分类号:DF795.2 文献标志码:A 文章编号:1008-3650(2015)05-0422-02 doi: 10.16467/j.1008-3650.2015.05.018
Genetic Polymorphism of 23 STR Loci of Kazak Population in Hotan Area
LIU Sheng1, JIA Fei2, LIU Feng2,*
1.Wafangdian Public Security Bureau, Liaoning Dalian 116300, China
2. Forensic Science Division of Liaoning Provincial Public Security Bureau, Shenyang 110032, China
Abstract

The genetic polymorphism of 23 short tandem repeat (STR) loci of 1130 unrelated Kazak individuals in Hotan area of Xinjiang, was investigated with GoldeneyeTM24A PCR amplification Kit and the population genetics parameters were calculated with PowerStats statistic software. 22 loci were found no deviation from Hardy-Weinberg equilibrium (P>0.05) except D13S317 locus. Matching probability (PM) ranged from 0.0113 to 0.2035 together with the power of discrimination (DP) 0.7965 to 0.9887, polymorphism information content (PIC) 0.5688 to 0.9208, power of exclusion (PE) 0.3355 to 0.8408, heterozygosity (He) 0.6354 to 0.9221. The total discrimination power (TDP) and the combined power of exclusion (CPE) of 23 loci were 1-4.53×1029 and 0.999 999 999 804, respectively. The 23 STR loci were highly polymorphic in Kazak population of Hotan area. The polymorphic information would serve as reference data for forensic personal identification and paternity testing.

Keyword: forensic biological evidence; STR loci; allelic frequencies; polymorphism; Kazak population

本文采用GoldeneyeTM24A基因分型系统(中国基点认知公司)对D13S317、TPOX等23个STR基因座在新疆和田地区哈萨克族人群遗传学数据进行调查分析。选取1130例无关个体血样, 其中, 女性177例, 男性953例。共检出286种等位基因, 等位基因及等位基因频率分布见表1。经χ 2检验, 除D13S317基因座(P=0.0030)外, 22个基因座符合Hardy-Weinberg平衡(P> 0.05)。有文献报道少数民族群体STR基因座频率调查发现个别基因座分布偏离Hardy-Weinberg平衡 [1, 2], 原因可能一是基因分型分析出错, 二是群体出现杂交, 三是群体中某个稀有等位基因纯合子的数目多于杂合子。本例中发生偏离提示可能来自群体杂交, 造成种族不纯, D13S317基因座频率可通过亚种族校正系数来修正[3, 4]。采用PowerStats 软件(美国Promega公司)分析处理数据, 获得群体遗传学数据(见表2)。按照郑秀芬[1]的方法, 计算23个STR基因座的累积个体识别能力(total probability of discrimination power, TDP)达1-4.53× 1029, 累积非父排除率(cumulative power of exclusion, CPE)达0.999 999 999 804。在新疆和田地区哈萨克族群体中有较高法医学个人识别和亲子鉴定应用价值。

表 1 新疆和田地区哈萨克族23个STR基因座等位基因频率分布(n=1130) Table 1 Allele frequencies of 23 loci of Kazak population in Hotan area, Xinjiang (n=1130)
表 2 23个STR基因座在新疆和田地区哈萨克族人群中的群体遗传学指标(n=1130) Table 2 The population genetic parameters of 23 loci of Kazak population in Hotan area, Xinjiang (n=1130)

The authors have declared that no competing interests exist.

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