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周晶, 牛一平, 杜潇. .石家庄地区汉族人群18个STR基因座遗传多态性调查[J]. 刑事技术,2015,40(3): 244-245
ZHOU Jing, NIU Yiping, DU Xiao. .Genetic Polymorphism of 18 STR Loci of Han Population in Shijiazhuang[J]. Forensic Science and Technology,2015,40(3): 244-245
10.16467/j.1008-3650.2015.03.019  
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《刑事技术》编辑部
石家庄地区汉族人群18个STR基因座遗传多态性调查
周晶, 牛一平, 杜潇
石家庄市公安局物证鉴定所,石家庄050021

作者简介：周 晶（1976—）,女,河北临西人,副主任法医师 ,学士,研究方向为法医遗传学。 E-mail:zjwy2004@126.com

摘要

目的 调查石家庄汉族人群18个STR基因座的遗传多态性。方法 应用DNA TyperTM 15 Plus荧光标记复合扩增系统检测323名石家庄地区汉族无关个体18个STR基因座多态性,计算群体遗传学参数。结果 18个STR基因型分布均符合Hardy-Weinberg平衡（P>0.05）,共检出198个等位基因, 695个基因型,12个微变异等位基因。结论 18个STR基因座在石家庄汉族人群中有较高的遗传多态性。

关键词: 法医遗传学; STR基因座; 遗传多态性; 石家庄地区; 汉族
doi: 10.16467/j.1008-3650.2015.03.019
Genetic Polymorphism of 18 STR Loci of Han Population in Shijiazhuang
ZHOU Jing, NIU Yiping, DU Xiao
Institute of Forensic Science, Shijiazhuang Public Security Bureau, Shijiazhuang 050021,China
Abstract

Objective To investigate genetic polymorphism of 18 STR loci on human chromosomes of Han population in Shijiazhuang.Methods Allelic frequencies for 18 STR loci and genetic parameters for forensic purpose were calculated.Results No deviations of allelic frequency from Hardy-Weinberg equilibrium expectations were observed (P>0.05). 198 alleles and 695 genotypes were gained. Allele frequencies varied between 0.002 and 0.545; heterozygosity (H) between 0.624 and 0.932; matching probability (Pm) between 0.016 and 0.189; power of discrimination (DP) between 0.811 and 0.984; polymorphism information content (PIC) between 0.580 and 0.910; power exclusion (PE) between 0.321 and 0.861. 12 off-ladder alleles of 8 STR loci were found.Conclusions 18 STR loci of Han population in Shijiazhuang are with relatively high polymorphism, and suitable for forensic individual identification, paternity testing, and relative research.

Keyword: forensic genetics; STR loci; genetic polymorphism; Shijiazhuang area; Han population

应用DNA TyperTM 15 Plus扩增试剂盒（公安部物证鉴定中心）对石家庄地区323名汉族无关人群18个基因座(CSF1PO、D2S1338、D3S1358、D5S818、D6S1043、D7S820、D8S1179、D12S391、D13S317、D16S539、D18S51、D19S433、D21S11、FGA、PentaE、TH01、TPOX、vWA)进行调查, 用Modified-Powerstates分析软件进行统计学计算[1], 应用Hardy-Weinberg平衡软件对各个基因座数据进行χ 2检验[2], 获得了该地区汉族群体遗传学数据（见表1、表2）。

表 1 石家庄汉族人群18个STR基因座法医学遗传参数(n=323) Table 1 Genetic parameters of 18 STR loci of Han population in Shijiazhuang (n=323)
表 2 石家庄地区汉族人群18个STR基因座等位基因频率 (n=323) Table 2 Allele Frequency of 18 STR loci of Han population in Shijiazhuang (n=323)

18个STR基因座, 共检测到198个等位基因, 其频率在0.002~0.545之间, 共695个基因型。在8个基因座上检出12个微变异等位基因（OL）, 其频率均小于0.005（见表3）, 对于法医的个体识别及亲子鉴定具有一定的意义。各基因座的H值分布在0.624~0.932之间, DP值0.811~0.984, PE值0.321~0.861, PIC值0.580~0.910, 累计匹配率为5.736× 10-22, 累计个体识别率达到0.999 999 999 999 999 999 999 426, 三联体的累计非父排除概率0.999 999 973 339。

表 3 石家庄地区汉族人群18个STR基因座的微变异等位基因情况 Table 3 Variant alleles of 18 STR loci of Han population in Shijiazhuang

The authors have declared that no competing interests exist.

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