目的利用法医DNA标准品对自主研发的法医DNA检验试剂耗材进行电泳检测准确性的实验考查。方法实验平台分为2个:平台1全部由美国Applied Biosystems公司进口的3130xl遗传分析仪、16道电泳毛细管与甲酰胺、电泳缓冲液、凝胶构成;平台2由Applied Biosystems公司进口的3130xl遗传分析仪、16道电泳毛细管与本实验室研发的甲酰胺、电泳缓冲液、凝胶构成。在实验平台上对allelic ladder、内标等标准品以及阳性对照9947a的STR复合扩增产物进行电泳检测,利用GeneMapper软件对电泳结果进行分析。结果自主研发的法医DNA检验试剂耗材可与国外进口的3130xl遗传分析仪配套使用,构建的实验平台对法医DNA标准品的检测结果准确无误。结论自主研发的甲酰胺、电泳缓冲液、凝胶等法医DNA检验试剂耗材检测准确性达到了国外同类产品水平。

Objective To study the accuracy of reagents for DNA electrophoresis using forensic DNA standard samples. Methods Two experiment platforms were involved in the detection systems, one was made up of 3130xl genetic analyzer, capillary array and the reagents for DNA electrophoresis including formamide, buffer and polymer from Applied Biosystems Inc. the other was made up of 3130xl genetic analyzer, capillary array from Applied Biosystems Inc. and the reagents developed by our own. Forensic DNA standard samples such as allelic ladder, internal standard DNA and the PCR products of positive control 9947a were detected on the experiment platforms. The results were analyzed by GeneMapper software.Results The formamide, buffer and polymer developed by our own can be compatible with 3130xl genetic analyzer. The detection results of forensic DNA standard samples were accurate. Conclusion The reagents for DNA electrophoresis developed by our own are of the same accuracy level as the similar foreign products.