TU Guozhang, ZHANG Xianqiang, XIAN Dexin
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Objective To explore the effective preservation time for drunk driver's blood to receive valid detection so as to ensure the accuracy of alcohol concentration determined. Methods Three kinds of vacuum anti-coagulative blood samples, kept respectively in the tube of EDTA-K2, sodium citrate (1:9) and sodium heparin, were selected to preserve under -20℃. Alcohol concentration of the blood samples was detected with gas chromatography (GC) on the day of 0, 3, 7, 14, 21, 28, 42 and 49 for primary screening. Into the tube showing the best stability of alcohol content was stored the venous blood at -20℃. GC was once again used to determine the alcohol concentration of the stored blood at the day of 0, 3, 7, 14, 21, 30, 60, 90, 120, 150 and 180. Results The EDTA-K2 tube showed of the best stability of blood alcohol concentration while both the sodium citrate (1:9) and sodium heparin tube made the alcohol content changed obviously after 42 days. In the EDTA-K2 tube, the alcohol kept its concentration steadily within 90 days, yet then being significantly continuous decreasing. Conclusions The preservation chronergy is within 90 days for the drunk driver's blood sample to be kept with EDTA-K2 tube.