GAO Linlin, YAN Jiangwei, TANG Hui, et al.
Forensic Science And Technology. 2006, 0(6): 27.
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ObjectiveTo establish the method of screening mtDNA heteroplasmy by DHPLC, and to investigate the frequency of mtDNA heteroplasmy in control region within multiple tissues of the same individual, it is provided reference by use of mtDNA technology in forensic community. MethodSelect blood, heart, liver, spleen, lung, kidney,pancreas,brain,muscle,skin,rib,nail and hair of 18 individuals, screening the heteroplasmyic samples by DHPLC,and confirm by direct sequencing. Result9 heteroplasmic individuals were detected,the frequency of heteroplasmy is highest in muscle tissue. ConclusionIt is important to take into account mtDNA heteroplasmy in forensic applied field.