JIANG Chengtao, YE Jian, ZHAO Xingchun,et al.
Forensic Science And Technology. 2007, 0(5): 7.
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A study of 18loci multiplex was carried out on the coamplification of seventeen STR loci plus the genderdetermining locus Amelogen in a single amplification/detection of all CODIS (Combined DNA Index System) STR loci (CSF1PO,D3S1358,D5S818,D7S820,D8S1179,D13S317,D16S539,D18S51,D21S11,FGA,TH01,TPOX,and vWA) as well as four widelyaccepted STRs (D6S1043,PentaE,PentaD and D2S1338).By using fivedye fragment analysis technology and previously optimized DNATyperTM15 kit primer sequences, the results showed the multiplex sucessfully coamplify and simultaneously detect all 18 loci and the multiplex could be a useful tool for forensic evidence testing.