CHEN Hu1, ZHU Shaohua, FANG Dong, et al.
Forensic Science And Technology. 2007, 0(2): 15.
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ObjectiveTo observe the relationship between the changes of corneal aquiporin1(AQP1) and postmortem interval (PMI). Methods50 healthy rabbits were divided randomly into 10 groups according to the time of 0h, 6h,12h, 24h, 36h 48h, 60h, 72h, 84h and 96h after the death caused by air embolism and the cornea was dissected. The corneal AQP1 was observed by the method of immunohistochemical staining and analyzed by a image analyze system. the parameters of positive area (A)、integral optical density(IOD)、average optical density(AOD) and average gray(AG) was used to measure the content changes of corneal AQP1 after different PMI. ResultsThe positive reaction of corneal AQP1 was showed a timedependent decrease until 84h after death. and the parameters of A, IOD, AOD and AG were also showed a decline regularly. ConclusionThe content of corneal AQP1 was changes regularly after death, and the parameters of A, IOD, AOD and AG could be used as new indexes for estimating the PMI.