01 October 2008, Volume 0 Issue 5

    

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  Preliminary study on extraction and examination of DNA in Yunnan Cannabis

  PEI Li，NIU Huiyuan，MAO Zeshan，et al.

  Forensic Science And Technology. 2008, 0(5): 3.

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  ObjectiveTo develop a method for the extraction and examination of total DNA of Yunnan Cannabis. MethodThe improved isolation method of SDS were used to extract the total DNA of Cannabis, and DNA were examined by PCR. ResultsThe DNA polymorphism has been examined successfully by the cannabis sativa special primers which had been screened. ConclusionThe DNA of cannabis can be extracted and detected efficiently by this method, which provides the basis for the identification of different species of cannabis.
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  Data analysis of printing paper measured by XRF

  GUO Hongling, QUAN Yangke, TAO Keming，et al

  Forensic Science And Technology. 2008, 0(5): 6.

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  XRF and XRD were used to analyze 21 samples of printing paper. The results were processed by correlation and cluster analysis. The combined analysis showed that this kind of method can be used to discriminate printing paper nondestructively.
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  Identification of accelerant in fire debris by automatic thermal desorption system and gas chromatographymass spectrometry

  DIAO Zhongwen,XING Ruokui，WANG Songcai，et al.

  Forensic Science And Technology. 2008, 0(5): 10.

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  ObjectiveTo present a method for accelerant gasoline identification in fire debris. MethodIgnitable liquid residues from fire debris samples were adsorbed and concentrated by automatic thermal desorption system, then analysed by gas chromatographymass spectrometry. ResultThe target compounds of gasoline were detected. ConclusionThe means is simple, convenient and practical.
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  The determination of Diazepam by GC-CI-MS/MS

  LUO Fang,WANG Qinghua, LIU Ying

  Forensic Science And Technology. 2008, 0(5): 13.

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  In this article, GCCIMS/MS was applied to analyze the trace Diazepam in biological tissue .When the parent ion was m/z 285 and the exact CID voltage was 0.8v,the result was satisfactory. The GC/MS/MS has many advantages, such as eliminating the disturbance, fast, high sensitive and accurate.
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  Determination of Bromadiolone in whole blood by HPLC-ESI-MS/MS

  ZHAO Haiyu, SONG Ming

  Forensic Science And Technology. 2008, 0(5): 16.

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  ObjectiveTo establish a HPLCESIMS/MS method for determination of Bromadiolone in whole blood. MethodsAfter Bromadiolone in whole blood were extracted by ethyl acetate， the samples were separated on Restek Ultra C18 (150 mm×2.1 mm×5μm) at a constant temperature of 23℃ by using CH3OH as a mobile phase .The flow rate was 200μl/min. Detection was performanced on an electrospray ionization ( ESI) in the negative mode. ResultsCalibration curves were linear within the range of 0.1～50mg/land the limit of quantitation was found to be 0.028ng. The extraction recovery were 94.34% and the RSD were 7.3%. ConclusionThis method is simple， sensitive and accurate for the determination of Bromadiolone in poisoncases.
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  Preservation of ninhydrin treated paper

  HUANG Wei，YU Tao，YANG Zhicheng

  Forensic Science And Technology. 2008, 0(5): 19.

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  The colour of paper sheet which was treated by ninhydrin for developing fingerprint will change after a period of time. In this paper, we studied the preservation conditions of ninhydrin treated paper sheet experimentally.
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  A study on the method to screen toxicants automatically by GC/MS

  LI Qian, DAI Weilie,WEN Jinfeng, et al

  Forensic Science And Technology. 2008, 0(5): 23.

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  To found a quick and sensitivitive method to screen familiar toxicants.MethodsA user's library of spectrum of familiar toxicants was build up and a optical condiction to separate and analyze the toxicants was selected. ResultsA user's library of spectrum containing 1306 toxicants has been built; a method to screen pesticides automatically has been edited;the RT and detection limit of 150 toxicants have been attained; ConclusionThe method is quick sensitive and reliable. It can take place of the manual operation of MS searching.
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  Identification of testament handwriting

  JIA Zhihui

  Forensic Science And Technology. 2008, 0(5): 27.

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  By analysis and comparison of typical cases, the characteristics of testament handwriting were summarized and the key point of examination were presented.
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  Application of faintness clustering for 　verifying portrait

  XIANG　Shuzhen

  Forensic Science And Technology. 2008, 0(5): 30.

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  ObjectiveTo explore the method of verifying portrait with the faintness clustering analysis. MethodUse the faintness clustering analysis in the portrait　verifying to classify the samples, confirm the clustering state under the given condition and obtain a practical classification. ResultFaintness clustering analysis can verify the portrait classification effectively. ConclusionThis method has certain practicality for casework.
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  The nuclear acid fingerprinting analysis in bioforensics

  MA Rongliang

  Forensic Science And Technology. 2008, 0(5): 33.

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  Currently nuclear acid fingerprinting analysis is one of the most useful approaches in bioforensics or microbial forensics， which is an entirely new discipline to detect the properties and resources of bioagents used in biocrimes. This paper describes the principle, procedure, characteristics　and the actual application of VNTR/MLVA、PFGE、AFLP、SNP methods in nuclear acid fingerprinting techniques. Some suggestions to develop forensics in China are put forward.