25 June 2012, Volume 37 Issue 3
    

  • Select all
    |
    Orginal Article
  • Analysis of nonbenzodiazepine hypnotic in biology samples by ASE and GPC-GC
    ZHANG Lei-ping,SHU Cui-xia,DU Hong-yan
    Forensic Science And Technology. 2012, 37(3): 6-8.
    Abstract ( ) Download PDF HTML   Knowledge map Save
    Objective To develop ASE-GPC method for analysis of nonbenzodiazepine hypnotic in biology samples. Methods The nonbenzodiazepine hypnotic was extracted with accelerated solvent extraction, cleaned by auto gel permeation chromatography and then the collected solution was analyzed by GC/NPD. Results The recovery rate is 83%, the limits of detection is 0.05~0.1μg·mL-1. Conclusion ASE and GPC method can be used in forensic toxicological analysis.
  • Application of real-time quantitative PCR (SYBR Green I) technique in forensic science
    SHI Yue-qun,HU Lan
    Forensic Science And Technology. 2012, 37(3): 9-12.
    Abstract ( ) Download PDF HTML   Knowledge map Save
    Objective To apply the real-time quantitative PCR assay with the SYBR Green I to DNA analysis in forensic science. Methods DNA abstracted from variety of biological samples encountered in forensic science were quantitated by using the SYBR Green I, and the subsequent procedures of STR testing were then conducted. Results The quantity of DNA of variety of biological samples was obtained. Conclusion Real-time quantitative PCR is useful technique for DNA analysis in forensic science.
  • Development of PCR enhancer for direct amplification of samples on FTA cards
    ZHAO Xing-chun,JIANG Bo-wei,YE Jian
    Forensic Science And Technology. 2012, 37(3): 13-15.
    Abstract ( ) Download PDF HTML   Knowledge map Save
    Objective To validate the PCR enhancer for direct amplification of forensic DNA database samples stored on FTA cards. Methods Direct amplification on FTA punches were performed based on the use of commercialized STR amplification kit. Results High quality of STR profiles were obtained from described samples above.Conclusion The newly developed PCR enhancer can be applied to the area of forensics.
  • The preliminary survey of diatom in winter around Beijing
    REN He,ZHANG Xu,LIU Guo-wei,et al
    Forensic Science And Technology. 2012, 37(3): 16-19.
    Abstract ( ) Download PDF HTML   Knowledge map Save
    Objective To investigate the genera and species of diatom in winter around Beijing city.Methods In winter,seventeen water samples were collected from five rivers and seven separated water sites,the diatom were analyzed with microscope.Results 12 species of 3 genera diatom were found from five rives,11 species of 7 genera were found from seven separated water sites.Conclusion Diatom analysis is an effective tool for diagnose drowning.
  • Extraction of organophosphorus pesticides in human blood using accelerated solvent extraction method
    DU Hong-yan,DONG Ying,ZHANG Lei-ping,et al
    Forensic Science And Technology. 2012, 37(3): 20-21.
    Abstract ( ) Download PDF HTML   Knowledge map Save
    Objective To develop accelerated solvent extraction methods for determining organophosphorus pesticides in blood sample. Methods Organophosphorus pesticides were extracted by accelerated solvent extraction method with optimized parameters, and purified by the alumina powder online. Concentrated elution was analyzed with GC/FPD. Results The recoveries of organophosphorus pesticides were larger than 70%. A good linear relationship was obtained at the concentration range of 0.05μg/mL~5μg/mL. The limit of detection was prior than 20ng/mL for signal to noise ratio(S/N) being 10.Conclusion The proposed method can be used to extract organophosphorus pesticides in biological specimens due to its superiority comparing with other traditional extracting methods.
  • Determination of hydrogen sulfide in blood by GC/MS
    QIAO Jing,YANG Shi-yun
    Forensic Science And Technology. 2012, 37(3): 22-23.
    Abstract ( ) Download PDF HTML   Knowledge map Save
    Objective To establish an analytical method of hydrogen sulfide(H2S) in blood by GC/MS.Methods NaCl (1g), water (3mL~5mL) and HCl (1mL) were mixed with blood sample (3mL~5mL) in head space bottle and analyzed by GC/MS.Results H2S in blood sample was detected and reference retention time was 3.6minutes. Conclusion This method can be used in casework.
  • Analysis of 1-phenyl-1-propanone and 1-phenyl-2-propanone by GC/MS
    QIAN Zhen-hua,XU Peng
    Forensic Science And Technology. 2012, 37(3): 24-26.
    Abstract ( ) Download PDF HTML   Knowledge map Save
    In this paper, the analysis procedure of 1-phenyl-1-propanone and 1-phenyl-2-propanone by GC/MS was introduced. The chromatographic characteristic, use and control on law of 1-phenyl-1-propanone and 1-phenyl-2-propanone were described.
  • Genetic polymorphisms of 15 STR loci of Africa population living in Guangzhou
    LIU Hong,LIU Chang-hui,HU Lan,et al
    Forensic Science And Technology. 2012, 37(3): 27-30.
    Abstract ( ) Download PDF HTML   Knowledge map Save
    Objective To study the genetic polymorphism of 15 autosomal short tandem repeat(STR)(FGA、vWA、CSF1PO、D3S1358、D5S818、D7S820、D8S1179、D13S317、D16S539、D18S51、D21S11、D2S1338、D19S433、D6S1043、D12S391)loci of the Africa population living in Guangzhou city.Methods 122 unrelated individuals of the Africa population were tested by using SinofilerTM kit,genetic polymorphisms data was analyzed by Powerstats V12、Genopop 3.4、Mega 3.4 software. Results The combined discrimination power(DP) and power of exclusion (PE) is 1-5.80147×10-18 and 1-8.6019×10-8, the Africa population living in Guangzhou city data denotes significant differences for the native Han population in Guangzhou, and is close to the Africa population in Bahamas. Conclusion The SinofilerTM kit is suitable for the Africa population living in Guangzhou in forensic personal identification and paternity test.Furthermore, this population was compared to the native Han population in Guangzhou, showing significant values in the racial distinction.
  • DNA test of blood fingerprint treated with fingerprints reagent EOS
    LIU Feng,LI Zai-er,XIAO Bin,et al
    Forensic Science And Technology. 2012, 37(3): 31-33.
    Abstract ( ) Download PDF HTML   Knowledge map Save
    Objective To investigate the effect of fingerprint reagent EOS to DNA test and explore methods for testing DNA from blood fingerprints treated with EOS.Methods The blood samples treated by EOS were wiped with water, wiped with alcohol, and scraped with scalpel, then the DAN were analyzed with ABI 3100 Genetic Analyzer and the results were compared. Results STR loci were genotyped successfully in the sample which was scraped and alcohol treated.Conclusion Scraping the samples and then treating with alcohol is the best way to extract DNA from the blood fingerprint treated by reagent EOS.
  • Determination of Cyanogene Fenvalerate in pond water by solid adsorption GC/MS
    LIU Min,ZHANG Shi-nan,YIN Juan
    Forensic Science And Technology. 2012, 37(3): 34-36.
    Abstract ( ) Download PDF HTML   Knowledge map Save
    Objective To explore a method for determining trace pesticide in poisoning water of fish ponds.Methods The absorbent GDX-403 was added to a water samples and the extraction was analyzed with GC/MS. Results Recovery rate of Cyanogene Fenvalerate was 90%, relative deviation was 5%, detection limit was 0.2 ppb. Conclusion The method is sensitive and can be used in casework.
  • Prediction of human externally visible characteristics based on DNA
    ZHAO Wen-jie,DENG Shu-jiao,HUANG Gui-qing,et al
    Forensic Science And Technology. 2012, 37(3): 37-41.
    Abstract ( ) Download PDF HTML   Knowledge map Save
    We aim to describe the forensic motivations for DNA-based prediction of human externally visible traits as well as the scientific challenges of finding predictive DNA markers,and will discuss examples with promising(e.g.sex,eye color and hair color),as well as less promising expectations(e.g.adult body height,human facial morphology).It offers some references for further study.
  • Progress in the study of estimation of age from pubic symphsis
    WANG Fu-lei,WANG Gang,TIAN Xue-mei,et al
    Forensic Science And Technology. 2012, 37(3): 42-45.
    Abstract ( ) Download PDF HTML   Knowledge map Save
    In this article, the methods for age estimation from pubic symphysis were reviewed and the research trends were analyzed.

Author Center

Review Center

Journal Online

京ICP备09053967号-3
Copyright © Forensic Science and Technology, All Rights Reserved.
Tel: 010-66269489，010-83752624　E-mail: xsjsbjb@163.com
Powered by Beijing Magtech Co., Ltd.