Objective To characterize the prepared diacetylmorphine hydrochloride reference material, and to evaluate the uncertainty of assessment of purity. Methods Diacetylmorphine hydrochloride was characterized by both quantitative-NMR (qNMR) and mass balance method. The organic impurities were identified by LC/MS-IT-TOF, and the contents of inorganic cations and anions, residual of volatile organic solvents and water in the reference material were assayed by ICP-MS, ion chromatography, Head-space GC-MS, Karl-Fischer titration Methods, respectively. Results The qNMR purity was calculated to be 95.6% with standard uncertainty of 0.13%, while the mass-balance purity was 95.3% with standard uncertainty of 0.93%. Conclusion The certified purity value of diacetylmorphine hydrochloride was 95.6%, with the expanded uncertainty of 1.2% (k=2).
Oil fingerprints on glass, plastic and paper were developed by SWP fluorescent agent and superglue. The Results indicated that SWP fluorescent agent is effective for oil fingerprint development and it can be used simultaneously with superglue.
Objective To investigate quantity and species distribution of diatomaceous in Wuhan central urban waters in spring. Methods 32 water samples were collected from rivers and lakes in Wuhan city. The OLYMPUS-DotSlide was used to obtain images of diatom and identify diatom species. Results 14 species of diatom was found and the diatom species contained in some rivers and lakes were different. Conclusion This study demonstrated the feasibility of digital virtual slice technology to identify diatoms and the database established can be used for investigation of drowning cases.
Objective To develop a rapid method for DNA analysis by using micro-cavity PCR chip. Methods By adding different concentrations of BSA solution and TaqGold polymerase in the PCR reaction buffer,the surface of the chip was optimized to achieve efficient amplification of the STR fragments. Results Complete STR genotyping Results were obtained with a concentration of 0.5mg/ml of BSA. Conclusion The optimized micro-cavity PCR chip was effective for multiplex amplification of STR fragments, and it is possible to achieve a rapid forensic DNA analysis at the crime scene through further optimization.
Objective To investigate the quantitative use of fat embolism by Sudan Ⅲ staining in forensic identification. Methods 11 cases of pulmonary embolism and sudden unexpected death with 11 cases of other causes were studied and the percentage of the positive area of fat embolism and the number of fat emboli were analyzed quantitatively. Results For the death coursed by pulmonary embolism, the percentage of positive staining area is (5±1.7) %, and the number of fat emboli is (9±2.4) %. A comparison with other causes of death has significant differences (P<0.01). Conclusion Quantitative Analysis of fat embolism in forensic identification is credible and practical.
Objective To investigate the effects of hand washing on detection of gunshot residues particles. Methods 24 experimenters fired with pistol were divided into four groups. The members of one group didn��t wash their hands, the members of other three groups washed their hands with water, washing liquid, and soap, respectively. After then, Gun shot residue samples were collected from all experimenters�� hands and examined by SEM/EDX. Results The average of gun shot residue particles for each group was 70, 37, 6 and 3. Conclusion Gun shot residue particles can be detected from washed hands, but the amount of particles will reduced after hand washing, especially the hands were washed with soap.
The automated DNA extraction system with magnetic beads is an automated DNA extraction instrument combined with magnetic separation technology. This article mainly introduces the basic composition, working principle, operation process and application in the forensic testing of the system, and looks into the future development of this system.
This paper reviewed the detection Methods of atropine, such as gas chromatography, high performance liquid chromatography and capillary electrophoresis.
Chemical derivatization of analyte is a very important process in the sample preparation. In this paper, the applications and progress of derivatization techniques in detection of Amphettamine were summarized.